Description
1. Membrane and Cytosol Protein Extraction Kit provides a simple and convenient way for extracting membrane and cytosol protein from cultured cells and tissues. This kit can extract not only the membrane protein on the plasma membrane, also the membrane protein on the Mitochondrial, Endoplasmic reticulum and Golgiapparatus.
2. This kit breaks cells properly by homogenizing, and through low speed centrifugation, discards nucleus and precipitate produced by the unbroken cells. Then, collect the supernatant and centrifuge at high speed to obtain the cell membrane pellets and the cytosol protein included supernatant. At last, extract the membrane protein from the pellets through Membrane Protein Extraction Reagent